Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration—In vitro study
Identifieur interne : 002C38 ( Main/Exploration ); précédent : 002C37; suivant : 002C39Effects of density of anisotropic microstamped silica thin films on guided bone tissue regeneration—In vitro study
Auteurs : Alejandro Pelaez-Vargas [Portugal, Colombie] ; Daniel Gallego-Perez [États-Unis] ; Angela Carvalho [Portugal] ; Maria H. Fernandes [Portugal] ; Derek J. Hansford [États-Unis] ; Fernando J. Monteiro [Portugal]Source :
- Journal of Biomedical Materials Research Part B: Applied Biomaterials [ 1552-4973 ] ; 2013-07.
Descripteurs français
- Wicri :
- topic : Biomatériau, Titane.
English descriptors
- KwdEn :
- Adhesion, Alkaline phosphatase, Anisotropic, Anisotropic microstamped silica, Anisotropic silica patterns, Anisotropic surface microtextures, Anisotropic topographies, Bioactive coatings, Biomaterials, Biomed, Biomed mater, Biomedical materials research, Bone cell responses, Bone cells, Bone integration, Bone tissue, Cell activity, Cell alignment, Cell culture experiments, Cell cultures, Cell growth, Cell nuclei, Cell proliferation, Cell responses, Cell shape, Ceramic implants, Clin implant dent relat, Contact angle, Contact angle measurements, Contract grant sponsor, Dataphysics instruments gmbh, Dent, Dental implants, Differential responses, Focal adhesions, Glass coverslips, Histomorphometric study, Human cells, Implant, Implant surface, Mater, Micropatterned, Micropatterned surfaces, Microtextured silica, Microtextures, Nuclei alignment, Oral maxillofac implants, Pdms stamp, Prosthet dent, Research report figure, Silica, Silica surfaces, Small bands, Soft techniques, Statistical analysis, Surface features, Surface microfeatures, Surface microtexture, Surface topography, Tcps controls, Tissue integration, Titanium, Titanium implants, Uorescence microscopy, Wiley periodicals.
- Teeft :
- Adhesion, Alkaline phosphatase, Anisotropic, Anisotropic microstamped silica, Anisotropic silica patterns, Anisotropic surface microtextures, Anisotropic topographies, Bioactive coatings, Biomaterials, Biomed, Biomed mater, Biomedical materials research, Bone cell responses, Bone cells, Bone integration, Bone tissue, Cell activity, Cell alignment, Cell culture experiments, Cell cultures, Cell growth, Cell nuclei, Cell proliferation, Cell responses, Cell shape, Ceramic implants, Clin implant dent relat, Contact angle, Contact angle measurements, Contract grant sponsor, Dataphysics instruments gmbh, Dent, Dental implants, Differential responses, Focal adhesions, Glass coverslips, Histomorphometric study, Human cells, Implant, Implant surface, Mater, Micropatterned, Micropatterned surfaces, Microtextured silica, Microtextures, Nuclei alignment, Oral maxillofac implants, Pdms stamp, Prosthet dent, Research report figure, Silica, Silica surfaces, Small bands, Soft techniques, Statistical analysis, Surface features, Surface microfeatures, Surface microtexture, Surface topography, Tcps controls, Tissue integration, Titanium, Titanium implants, Uorescence microscopy, Wiley periodicals.
Abstract
The growing demand for better implant aesthetics has led to increased research on the development of all‐ceramic dental implants. The use of microtextured coatings with enhanced properties has been presented as a viable way to improve tissue integrability of all‐ceramic implants. The aim of this study was to evaluate the effects of different densities of anisotropic microtextured silica thin films, which served as a model coating, on the behavior of human osteoblast‐like cells. The differential responses of human osteoblast‐like cells to anisotropic silica microtextures with varying densities, produced via a combination of sol–gel and soft lithography processing, were evaluated in terms of alignment, elongation (using fluorescence microscopy), overall cellular activity, and the expression/activity levels of alkaline phosphatase (ALP). Statistical analysis was conducted using one‐way ANOVA/Tukey HSD post hoc test. The thin films were thoroughly characterized via scanning electron microscopy/energy dispersive spectroscopy, Fourier transform infrared, and contact angle measurements. Thin film characterization revealed increased nanoscale roughness and reduced wettability on the micropatterned surfaces. Cell culture experiments indicated that the microtextures induced cell alignment, elongation, and guided colonization on the surface. Cells cultured on denser micropatterns exhibited increased metabolic activity (t = 14–21 days). The early expression/activity levels of ALP released into the medium were found to be significantly higher only on the least dense micropattern. These results suggest the possibility that microstructured silica thin films could be used to guide and enhance peri‐implant cell/tissue responses, potentially improving tissue integration for metallic and all‐ceramic dental implants. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Url:
DOI: 10.1002/jbm.b.32879
Affiliations:
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Fernandes</name><affiliation wicri:level="1"><country xml:lang="fr">Portugal</country><wicri:regionArea>Laboratório de Farmacologia e Biocompatibilidade Celular, Faculdade de Medicina Dentária, Universidade do Porto, Porto</wicri:regionArea><wicri:noRegion>Porto</wicri:noRegion></affiliation></author><author><name sortKey="Hansford, Derek J" sort="Hansford, Derek J" uniqKey="Hansford D" first="Derek J." last="Hansford">Derek J. Hansford</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Biomedical Engineering, The Ohio State University, Columbus</wicri:cityArea></affiliation></author><author><name sortKey="Monteiro, Fernando J" sort="Monteiro, Fernando J" uniqKey="Monteiro F" first="Fernando J." last="Monteiro">Fernando J. Monteiro</name><affiliation wicri:level="1"><country xml:lang="fr">Portugal</country><wicri:regionArea>INEB – Instituto de Engenharia Biomédica, Universidade do Porto, Porto</wicri:regionArea><wicri:noRegion>Porto</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Portugal</country><wicri:regionArea>Departamento de Engenharia Metalúrgica e Materiais, FEUP – Faculdade de Engenharia, Universidade do Porto, Porto</wicri:regionArea><wicri:noRegion>Porto</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Biomedical Materials Research Part B: Applied Biomaterials</title><title level="j" type="alt">JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B: APPLIED BIOMATERIALS</title><idno type="ISSN">1552-4973</idno><idno type="eISSN">1552-4981</idno><imprint><biblScope unit="vol">101B</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="762">762</biblScope><biblScope unit="page" to="769">769</biblScope><biblScope unit="page-count">8</biblScope><publisher>Wiley Subscription Services, Inc., A Wiley Company</publisher><pubPlace>Hoboken</pubPlace><date type="published" when="2013-07">2013-07</date></imprint><idno type="ISSN">1552-4973</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1552-4973</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adhesion</term><term>Alkaline phosphatase</term><term>Anisotropic</term><term>Anisotropic microstamped silica</term><term>Anisotropic silica patterns</term><term>Anisotropic surface microtextures</term><term>Anisotropic topographies</term><term>Bioactive coatings</term><term>Biomaterials</term><term>Biomed</term><term>Biomed mater</term><term>Biomedical materials research</term><term>Bone cell responses</term><term>Bone cells</term><term>Bone integration</term><term>Bone tissue</term><term>Cell activity</term><term>Cell alignment</term><term>Cell culture experiments</term><term>Cell cultures</term><term>Cell growth</term><term>Cell nuclei</term><term>Cell proliferation</term><term>Cell responses</term><term>Cell shape</term><term>Ceramic implants</term><term>Clin implant dent relat</term><term>Contact angle</term><term>Contact angle measurements</term><term>Contract grant sponsor</term><term>Dataphysics instruments gmbh</term><term>Dent</term><term>Dental implants</term><term>Differential responses</term><term>Focal adhesions</term><term>Glass coverslips</term><term>Histomorphometric study</term><term>Human cells</term><term>Implant</term><term>Implant surface</term><term>Mater</term><term>Micropatterned</term><term>Micropatterned surfaces</term><term>Microtextured silica</term><term>Microtextures</term><term>Nuclei alignment</term><term>Oral maxillofac implants</term><term>Pdms stamp</term><term>Prosthet dent</term><term>Research report figure</term><term>Silica</term><term>Silica surfaces</term><term>Small bands</term><term>Soft techniques</term><term>Statistical analysis</term><term>Surface features</term><term>Surface microfeatures</term><term>Surface microtexture</term><term>Surface topography</term><term>Tcps controls</term><term>Tissue integration</term><term>Titanium</term><term>Titanium implants</term><term>Uorescence microscopy</term><term>Wiley periodicals</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Adhesion</term><term>Alkaline phosphatase</term><term>Anisotropic</term><term>Anisotropic microstamped silica</term><term>Anisotropic silica patterns</term><term>Anisotropic surface microtextures</term><term>Anisotropic topographies</term><term>Bioactive coatings</term><term>Biomaterials</term><term>Biomed</term><term>Biomed mater</term><term>Biomedical materials research</term><term>Bone cell responses</term><term>Bone cells</term><term>Bone integration</term><term>Bone tissue</term><term>Cell activity</term><term>Cell alignment</term><term>Cell culture experiments</term><term>Cell cultures</term><term>Cell growth</term><term>Cell nuclei</term><term>Cell proliferation</term><term>Cell responses</term><term>Cell shape</term><term>Ceramic implants</term><term>Clin implant dent relat</term><term>Contact angle</term><term>Contact angle measurements</term><term>Contract grant sponsor</term><term>Dataphysics instruments gmbh</term><term>Dent</term><term>Dental implants</term><term>Differential responses</term><term>Focal adhesions</term><term>Glass coverslips</term><term>Histomorphometric study</term><term>Human cells</term><term>Implant</term><term>Implant surface</term><term>Mater</term><term>Micropatterned</term><term>Micropatterned surfaces</term><term>Microtextured silica</term><term>Microtextures</term><term>Nuclei alignment</term><term>Oral maxillofac implants</term><term>Pdms stamp</term><term>Prosthet dent</term><term>Research report figure</term><term>Silica</term><term>Silica surfaces</term><term>Small bands</term><term>Soft techniques</term><term>Statistical analysis</term><term>Surface features</term><term>Surface microfeatures</term><term>Surface microtexture</term><term>Surface topography</term><term>Tcps controls</term><term>Tissue integration</term><term>Titanium</term><term>Titanium implants</term><term>Uorescence microscopy</term><term>Wiley periodicals</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Biomatériau</term><term>Titane</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The growing demand for better implant aesthetics has led to increased research on the development of all‐ceramic dental implants. The use of microtextured coatings with enhanced properties has been presented as a viable way to improve tissue integrability of all‐ceramic implants. The aim of this study was to evaluate the effects of different densities of anisotropic microtextured silica thin films, which served as a model coating, on the behavior of human osteoblast‐like cells. The differential responses of human osteoblast‐like cells to anisotropic silica microtextures with varying densities, produced via a combination of sol–gel and soft lithography processing, were evaluated in terms of alignment, elongation (using fluorescence microscopy), overall cellular activity, and the expression/activity levels of alkaline phosphatase (ALP). Statistical analysis was conducted using one‐way ANOVA/Tukey HSD post hoc test. The thin films were thoroughly characterized via scanning electron microscopy/energy dispersive spectroscopy, Fourier transform infrared, and contact angle measurements. Thin film characterization revealed increased nanoscale roughness and reduced wettability on the micropatterned surfaces. Cell culture experiments indicated that the microtextures induced cell alignment, elongation, and guided colonization on the surface. Cells cultured on denser micropatterns exhibited increased metabolic activity (t = 14–21 days). The early expression/activity levels of ALP released into the medium were found to be significantly higher only on the least dense micropattern. These results suggest the possibility that microstructured silica thin films could be used to guide and enhance peri‐implant cell/tissue responses, potentially improving tissue integration for metallic and all‐ceramic dental implants. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.</div></front></TEI><affiliations><list><country><li>Colombie</li><li>Portugal</li><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><country name="Portugal"><noRegion><name sortKey="Pelaez Argas, Alejandro" sort="Pelaez Argas, Alejandro" uniqKey="Pelaez Argas A" first="Alejandro" last="Pelaez-Vargas">Alejandro Pelaez-Vargas</name></noRegion><name sortKey="Carvalho, Angela" sort="Carvalho, Angela" uniqKey="Carvalho A" first="Angela" last="Carvalho">Angela Carvalho</name><name sortKey="Carvalho, Angela" sort="Carvalho, Angela" uniqKey="Carvalho A" first="Angela" last="Carvalho">Angela Carvalho</name><name sortKey="Fernandes, Maria H" sort="Fernandes, Maria H" uniqKey="Fernandes M" first="Maria H." last="Fernandes">Maria H. Fernandes</name><name sortKey="Monteiro, Fernando J" sort="Monteiro, Fernando J" uniqKey="Monteiro F" first="Fernando J." last="Monteiro">Fernando J. Monteiro</name><name sortKey="Monteiro, Fernando J" sort="Monteiro, Fernando J" uniqKey="Monteiro F" first="Fernando J." last="Monteiro">Fernando J. Monteiro</name><name sortKey="Pelaez Argas, Alejandro" sort="Pelaez Argas, Alejandro" uniqKey="Pelaez Argas A" first="Alejandro" last="Pelaez-Vargas">Alejandro Pelaez-Vargas</name><name sortKey="Pelaez Argas, Alejandro" sort="Pelaez Argas, Alejandro" uniqKey="Pelaez Argas A" first="Alejandro" last="Pelaez-Vargas">Alejandro Pelaez-Vargas</name><name sortKey="Pelaez Argas, Alejandro" sort="Pelaez Argas, Alejandro" uniqKey="Pelaez Argas A" first="Alejandro" last="Pelaez-Vargas">Alejandro Pelaez-Vargas</name></country><country name="Colombie"><noRegion><name sortKey="Pelaez Argas, Alejandro" sort="Pelaez Argas, Alejandro" uniqKey="Pelaez Argas A" first="Alejandro" last="Pelaez-Vargas">Alejandro Pelaez-Vargas</name></noRegion></country><country name="États-Unis"><region name="Ohio"><name sortKey="Gallego Erez, Daniel" sort="Gallego Erez, Daniel" uniqKey="Gallego Erez D" first="Daniel" last="Gallego-Perez">Daniel Gallego-Perez</name></region><name sortKey="Hansford, Derek J" sort="Hansford, Derek J" uniqKey="Hansford D" first="Derek J." last="Hansford">Derek J. Hansford</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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